Agriculture
January 20, 2024
ToughStuff Desk Lamp Kit
Read SolutionImplemented by
ToughStuff International
Updated on March 8, 2024
·Created on August 31, 2021
Loop-mediated isothermal amplification (LAMP) based COVID-19 test. Courtesy of WHO Compendium 2021
The loop-mediated isothermal amplification (LAMP) based COVID-19 test uses viral RNA from heat-inactivated nasopharyngeal swab samples in an integrated modular centrifugal microfluidic platform. This method can deliver 20 test results via smartphone within 30 minutes. One device can perform up to 600 tests per day. This product is currently a prototype.
This product was selected for inclusion in WHO’s 2021 Compendium of Innovative Health Technologies for Low‐Resource Settings.
Target SDGs
SDG 3: Good Health and Well-Being
Market Suggested Retail Price
$500.00
Target Users (Target Impact Group)
Small and Medium-sized Enterprises, NGOs
Distributors / Implementing Organizations
None, as of 2020
Regions
Africa, Latin America, Mediterranean Sea Area
Manufacturing/Building Method
Potential to be mass-produced in location. The Disc requires either injection molding of plastic or hot embossing or CNC drilling capabilities and the reagents for the LAMP assay can be mass-produced.
Intellectural Property Type
Patent
Consumables
Lamp mastermix reagent
Detection sensitivity
~94%
Indispensable equipment for function (Y/N)
Yes
Maintenance or calibration required by user at time of use? (Y/N)
No
Number of Tests Performed
1
Power supply type: Continuous, Recharging only (V, time required, battery life), Other
Replaceable battery
Time required for procedure (minutes)
~30 minutes
Design Specifications
1. Collect swab sample into a transportation media; 2. Heat at 95 for 5 min to inactivate the sample (virus is now dead); 3. Take 1 microliter sample and add to LAMP mastermix (total volume = 10 uL) and pipette the solution into the microfluidics Disc channel and seal with a tape (Each disc can take 20 different samples); 4 Put the disc into the Hardware and push a button to start the isothermal reaction (programmed to stop after 30 min);6. Take a picture using smartphone (and analyse the image)
Using state of the art microfluidics, we have developed a LAMP based COVID test. In addition to precise fluid control, at the heart of our innovation is a novel agarose bead-based sample processing strategy to (1) stop the reaction and (2) improve the signal response of reaction (approx. 30x signal enhancement), thus allowing either visual by naked eye or smartphone-based detection. 1 uL swab sample is added to LAMP mastermix --> heat at 65 for 30 min --> readout using any smartphone.
The end-user takes swab sample,add to Lamp mastermix and and put into the channel, and push start button. After the reaction, the end-user uses a smart phone to take an image for analysis. Since the reaction is terminated automatically, the enduser do not need to make the image analysis directly
Uses rechargeable batteries
1 kg, 250 x 200 x 150 mm3
Replacement Components
Available separately
Lifecycle
5-10 years
Manufacturer Specified Performance Parameters
Reusable, cost-effective, and scalable
Vetted Performance Status
The method was first tested using extracted SARS-CoV-2 RNA for testing the assay specificity(specificity: 100% for SARS-CoV-2). Following, using clinical swab samples from Karolinska Hospital, the method was benchmarked against RT-qPCR (n =31), with proven sensitivity of 94% and specificity of 100%. Note, while the RT-qPCR was performed in centralised lab using standard procedure, our method takes heat-inactivated clinical sample and deliver results within 30 minutes; 1) We are planning to perform two pilot studies at resource-limited settings over the coming 6 months (in Ethiopia and Nigeria).
Complementary Technical Systems
Requires replaceable batteries to function as well as a clean water supply.
Academic Research and References
Soares, Ruben, et. al. “Point-of-care detection of SARS-CoV-2 in nasopharyngeal swab samples using an integrated smartphone-based centrifugal microfluidic platform“, medRxiv, Infectious Diseases, 2020
Compliance with regulations
None as of 2020
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